Journal article
Localisation-based imaging of malarial antigens during erythrocyte entry reaffirms a role for AMA1 but not MTRAP in invasion
DT Riglar, L Whitehead, AF Cowman, KL Rogers, J Baum
Journal of Cell Science | Published : 2016
DOI: 10.1242/jcs.177741
Abstract
Microscopy-based localisation of proteins during malaria parasite (Plasmodium) invasion of the erythrocyte is widely used for tentative assignment of protein function. To date, however, imaging has been limited by the rarity of invasion events and the poor resolution available, given the micron size of the parasite, which leads to a lack of quantitative measures for definitive localisation. Here, using computational image analysis we have attempted to assign relative protein localisation during invasion using wide-field deconvolution microscopy. By incorporating three-dimensional information we present a detailed assessment of known parasite effectors predicted to function during entry but a..
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Grants
Awarded by Pan-Massachusetts Challenge
Funding Acknowledgements
Experimental data presented here were made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. The research was directly supported by a National Health and Medical Research Council of Australia (NHMRC) Project Grant [APP1047085 to J.B.], Program Grant [637406 to A.F.C.] and a Human Frontier Science Program (HFSP) Young Investigator Program Grant [RGY0071/2011 to J.B.]. D.T.R. was supported during this work by a Pratt Foundation postgraduate scholarship through the University of Melbourne. A.F.C. is an International Scholar of the Howard Hughes Medical Institute. J.B. was supported through a Future Fellowship [FT100100112] from the Australian Research Council (ARC) and is currently supported by the Wellcome Trust through a New Investigator Award [100993/Z/13/Z]. Deposited in PMC for immediate release.